TEST
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SPECIMEN
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COLLECTION INSTRUCTIONS
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TRANSPORT
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Mycology Primary Specimen Identification
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Abscess
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Clean abscess surface with sterile saline or 70% alcohol.
Collect fluid/abscess material into clean tube and swab in Modified Amies Clear media (available at lab).
Fluid or abscess material is preferred over swab.
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Room temperature (15-30°C)
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Blood
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Collect 8-10 ml of blood drawn into a Myco F/Lytic bottle (available at lab). Transport to laboratory as soon as possible.
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Room temperature (15-30°C)
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CSF
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Collect 3-5 ml into sterile screw-cap tube.
Transport to laboratory within 24 hours.
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Room temperature (15-30°C)
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Ear
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Collect swab of infected area and transport in Modified Amies Clear media (available at lab).
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Room temperature (15-30°C)
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Hair
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Collect 5-10 hairs (and base of shaft) in clean tube or container, in paper envelope, or directly inoculated onto IMA media (Inhibitory Mold Agar).
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Room temperature (15-30°C)
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Mucocutaneous membranes
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Collect swab of infected area (e.g., mouth, vagina, urethra) and transport in Modified Amies Clear media (available at lab).
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Room temperature (15-30°C)
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Nails
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Clean nail with 70% alcohol. Scrape nail and discard. Scrape nail again from infected area and save in sterile container, paper envelope, or directly inoculated onto IMA media (Inhibitory Mold Agar).
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Room temperature (15-30°C)
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Respiratory
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Collect 7-10 ml of aerosol, early morning sputum, tracheal aspirate, lung biopsy, or bronchoscopy specimen in sterile container.
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Refrigerated (2-8°C)
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Skin
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Clean skin with 70% alcohol. Scrape lesion at the active margin but do not draw blood. Place scrapings in clean container or directly inoculate onto IMA media (Inhibitory Mold Agar).
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Room temperature (15-30°C)
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Swabs for C. auris surveillance
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Collect swab of axilla and groin area with BD Eswab Collection and Transport System with Modified Amies Clear medium (available at lab).
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Refrigerated (2-8°C)
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Tissue
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Submit in sterile container with a small amount of sterile saline.
Do not allow tissue to dry out.
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Room temperature (15-30°C)
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Urine
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Collect 25-30 ml of catheterized or early morning clean catch urine into a sterile container.
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Refrigerated (2-8°C)
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