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Escherichia coli (STEC) Culture

TEST NAMEEscherichia coli (STEC) Culture
DEPARTMENTBACT
TESTSB10
DESCRIPTIONScreening procedure for isolation and identification of Shiga toxin-producing Escherichia coli utilizing conventional biochemical, and whole genome sequencing techniques. 
Toxin production confirmed utilizing lateral flow immunoassay.
 
SPECIMEN REQUIREMENTS

SPECIMEN: Stool

CONTAINER: Stool transport bottles (Para Pak C&S), GN Broth or Rectal Swabs.
Note: The use of rectal swabs (Bacterial Culturettes) should be limited to patients with active disease, infants and children from whom feces may be difficult to obtain.
 

COLLECTION: The specimen of choice is the diarrheal stool collected during the acute stage of the disease. Keep the stool specimen cool, do not incubate or refrigerate. Portions containing blood or mucus usually contain the highest number of pathogens. 

TRANSPORT CONDITIONS: Room Temperature (25±5°C): 4 days (Preserved stool)

Refrigerated (5±3°C): Not recommended

Frozen (-15±5°C): Unacceptable for culture, acceptable for toxin assay only

CIDT Screens: 7 days

TURNAROUND TIME (TAT)

1 week

Culture 5 days

Toxin 1 day

REFERENCE RANGENegative
TEST METHODCulture
CPT CODES87046