| TEST NAME | Escherichia coli (STEC) Culture |
|---|---|
| DEPARTMENT | BACT |
| TESTS | B10 |
| DESCRIPTION | Screening procedure for isolation and identification of Shiga toxin-producing Escherichia coli utilizing conventional biochemical, matrix-assisted laser desorption ionization time of resolution (MALDI-TOF), and whole genome sequencing techniques. Toxin production confirmed utilizing lateral flow immunoassay. |
| SPECIMEN REQUIREMENTS | SPECIMEN: Stool CONTAINER: Stool transport bottles (Para Pak C&S), GN Broth or Rectal Swabs. COLLECTION: The specimen of choice is the diarrheal stool collected during the acute stage of the disease. Keep the stool specimen cool, do not incubate or refrigerate. Portions containing blood or mucus usually contain the highest number of pathogens. TRANSPORT CONDITIONS: Room Temperature (25±5°C): 4 days (Preserved stool) Refrigerated (5±3°C): Not recommended Frozen (-15±5°C): Unacceptable for culture, acceptable for toxin assay only CIDT Screens: 7 days |
| TURNAROUND TIME (TAT) | 1 week Culture 5 days Toxin 1 day |
| REFERENCE RANGE | Negative |
| TEST METHOD | Culture |
| CPT CODES | 87046 |
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